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Equal numbers of trophozoites were treated with 2 µM OZ439, 100 nM DHA, or no drug for 8 hours, then harvested for metabolites by extracting with 90% cold methanol containing the spike-in control, 0.5 µM [ 13 C 4 , 15 N 1 ]-Aspartate (Cambridge Isotope) that acts as an internal LC/MS standard to correct for technical variation arising from sample processing, as described previously ( 65 )
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The most meaningful interpretation is modest: it may help some dogs feel a bit more “ready” for their normal routine, especially when paired with conditioning and appropriate nutrition